Composite

Part:BBa_K3206008:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


Constitutively expressed GcvA gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 599
    Illegal BamHI site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part is made up of a constitutively expressing promoter (BBa_J23100), RBS (BBa_B0034), GcvA CDS (BBa_K3206004) and a terminator (BBa_B0015). The composite part was made compatible with Type IIs assembly. BsaI restriction sites were added and fusion sites were made compatible with a DVK-AF level-1 pOdd destination vector. This part was designed in benchling and ordered as a gBlock from IDT.

Source

Basic parts were all sourced from the iGEM Registry of Standard Biological Parts.

References

Wilson, R L & Stauffer, G V, 1994. DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system. The Journal of Bacteriology, 176(10), pp.2862–2868.